As a general rule, enzyme cleaners will do no more harm to the surface of the mattress than water would. Remove all bedding before application. For fresh urine deposits: Do not use any other cleaners or chemicals.
While vinegar, borax powder, and hydrogen peroxide are impressive cleaning agents, they are unfortunately not an enzyme cleaner. They do break down stains, but it’s not by using enzymes like protease and terpenes. Instead, it is the acidic nature of white vinegar that works to break down stains.
You can use a variety or mixture of citrus peels to make your homemade enzyme cleaner, including lemon, lime, grapefruit, and orange. It’s important to use fresh citrus peels that aren’t dried out or rotting. Dried peels won’t contain enough citrus oil for cleaning, and rotten ones will cause the mixture to mold.
One of the ingredients that helps OxiClean Laundry Detergent attack stains and smells so effectively is enzymes, specifically proteolytic enzymes. They work in much the same way the live enzymes work in Bac Out.
In both cases, enzymes are more effective than general cleaning with vinegar, which could leave a residue that might inhibit future cleaning attempts with enzymes.